Today, the advancement in life-sciences technology allows different types of nucleic acids to be transfected into mammalian cells, and these include Deoxyribonucleic acids (DNAs), Ribonucleic acids (RNAs) as well as small, non-coding RNAs such as siRNA, shRNA and miRNA ( Borawski et al., 2007 Yamano, Dai & Moursi, 2010 Sork et al., 2016 Shi et al., 2018). Besides, transfection can be employed as one of the strategies in gene therapy to treat incurable, inherited genetic diseases ( Yao et al., 2008 Yamano, Dai & Moursi, 2010 Tomizawa et al., 2013).
Understanding the molecular pathway of disease allows the discovery of specific biomarkers that may be applied to diagnose and prognose diseases ( Ye et al., 2017 Roser et al., 2018). For the past 30 years, transfection has gained increasing popularity due to its wide application for studying cellular processes and molecular mechanisms of diseases ( Arnold et al., 2006 Ishida & Selaru, 2012 Chow et al., 2016). Transfection is a process by which foreign nucleic acids are delivered into a eukaryotic cell to modify the host cell’s genetic makeup ( Kim & Eberwine, 2010 Chow et al., 2016).
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Abbreviations for transfection related chemicals/methods: AT: Attractene DAC: 3β- DC: 3β- DFT1: DharmaFECT1 DFT2: DharmaFECT2 DFT3: DharmaFECT3 DFT4: DharmaFECT4 DOTAP: DTP EF: ExpressFect EFT: Effectene EG500: ExGen 500 E5: Escort IV FGHD: FuGENE HD FG6: FuGENE 6 GJ: GeneJammer HPT: HiperFect IN: INTERFERin JP: JetPrime JPI: JetPEI LF: Lipofectin LP+ve: Lipofectamine plus LP2000: Lipofectamine 2000 LP3000: Lipofectamine 3000 LTX: Lipofectamine LTX Matra-A: Magnetic-assisted transfection NaF: Nanofectamine NFN: Nanofectin NF: Nucleofection OF: Oligofectamine PEI: Polyethylenimine RNAiMAX: Lipofectamine RNAiMAX SFT: SuperFect TF: TurboFect TKO: TransIT-TKO TLT1: TransIT-LT1 VF: ViaFect XG: X-tremeGENE XG-HD: X-tremeGENE-HD XG-9: X-tremeGENE-9 X2: TransIT-X2 293: TransIT-293 2020: TransIT-2020 “=”: Similar “>”: More superior.
Cell lines details: AGS: Human gastric adenocarcinoma cell line A549: Human adenocarcinomic alveolar basal epithelial cell A673: Human ewing sarcoma cell line A-10 SMCs: Rats smooth muscle cells bMDM: Bovine monocyte-derived macrophages C2C12: Mouse myoblasts C3H10T1/2: Mouse stem cells HAECs: Human aorta endothelial cells HASMCs: Human aorta smooth muscle cells hBM-MSC: Human bone marrow mesenchymal stem cells HCT116: Human colorectal carcinoma cell line HEK: Primary human epidermal keratinocytes HEK293: Human embryonic kidney cells 293 HeLa: Human cervical cancer cell Hep G2: Human hepatocellular carcinoma cells hiPSC-CMS: human induced pluripotent stem cells derived cardiomyocytes HL-60: Human leukemia cell line hPDLSCs: Human periodontal-ligament stem cells hTERT MSCs: Human telomerase reverse transcriptase mesenchymal stem cells Huh-7: Human liver cell line HUVECs: Human umbilical cord vein cells JU77: Human primary myoblast and human lung mesothelioma cells MCF-7: Oestrogen responsive breast cancer cell line MC3T3-E1: Mouse preosteoblasts MDA-MB-231: Triple negative breast cancer cell line MESCs: Sickle cell disease transgenic mice embryonic stem cell Neuro2a: mice neuroblastoma cells Panc 04.03: Human pancreas epithelial cancer cell line PECs: Primary endothelial cells PTE and THE: Primary pig and human tracheal epithelial cells PT-30: Human epithelial precancer cells P16: Primary embryonic pig fibroblasts REF: Embryonic rabbit ear fibroblasts SH-SY5Y: Human neuroblastoma cells SKOV3: Human ovarian cancer cell line SMA: Spinal muscular atrophy fibroblast cells UMR: Rat osteosarcoma cell line U87MG: human glioblastoma cells Z3: Embryonic zebrafish cell line 4T1: Mouse mammary carcinoma 16HBE14o- and CFBE41o-: Immortalized human bronchial epithelial cells. Supplemental Information 1: Comparison of the transfection efficiencies of commercially available transfection reagents and transfection conditions reported in different studies ( n = 20).
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